Is dihydrocoumarin toxic

Dihydrocoumarin, fragrance, used in food, also used as coumarin substitute, used as cosmetic flavor; Blend cream, coconut, cinnamon flavor; It is also used as a tobacco flavor.

Is dihydrocoumarin toxic

Dihydrocoumarin is not toxic. Dihydrocoumarin is a natural product found in yellow vanilla rhinoceros. It is prepared by hydrogenation of coumarin in the presence of nickel catalyst at 160-200 ℃ and under pressure. It can also be used as raw material, hydrolyzed in alkaline aqueous solution to produce o-hydroxyphenylpropionic acid, dehydration, closed-loop obtained.

Storage condition

Closed and dark, stored in a cool and dry place, the space in the barrel is as small as possible under safety permits, and is filled with nitrogen protection. Store in a cool, ventilated warehouse. Stay away from fire, water. Should be stored separately from oxidizer, do not mix storage. Equipped with the corresponding variety and quantity of fire equipment.

In vitro study

In vitro enzymatic assay, dihydrocoumarin induced concentration-dependent inhibition of SIRT1 (IC50 of 208μM). Reductions in SIRT1 deacetylase activity were observed even at micromolar doses (85±5.8 and 73± 13.7% activity at 1.6μM and 8μM, respectively). Microtubule SIRT2 deacetylase was also inhibited in a similar dose-dependent manner (IC50 of 295μM).

After 24 hours of exposure, dihydrocoumarin (1-5mM) increased cytotoxicity in TK6 cell lines in a dose-dependent manner. Dihydrocoumarin (1-5mM) increased apoptosis in TK6 cell lines in a dose-dependent manner at a 6-hour time point. A 5mM dose of dihydrocoumarin increased apoptosis at a 6-hour time point in the TK6 cell line. After a 24-hour exposure period, dihydrocoumarin (1-5mM) increased p53 lysine 373 and 382 acetylation in a dose-dependent manner in the TK6 cell line.